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Modifying Enzymes

Proteins that have the ability to structurally alter macromolecules without being altered themselves. Useful for a variety of molecular biology procedures; products include DNA and RNA polymerases, phosphatases, kinases, nucleases, etc.
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Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

EDGE ENCOMPASS_PREFERRED
92

Corning™ Dispase
GSA_VA GREENER_CHOICE

Bacillus-derived neutral metalloprotease that is recommended for recovering cells cultured on BD Matrigel™ Basement Membrane Matrix

EDGE
93

MP Biomedicals™ Hyaluronidase, Bovine Testes

Bovine Testes

Content And Storage -20°C
Inhibitors Fe2+ and Fe3+ are inhibitory as are Mn2+ and Cu2+
Form Lyophilized Powder
Product Type Hyaluronidase
Molecular Weight (g/mol) ≈55
Color Tan
pH 4.5 to 6
Concentration ≥300 U/mg
For Use With (Application) Drug dispersion and delivery
Source Bovine testes
94

MP Biomedicals™ alpha-Chymotrypsin from Bovine Pancreas

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains on carboxyl end of the bond

Content And Storage 0°C
Inhibitors Heavy Metals, natural Trypsin inhibitors to various degrees, Organophosphorus compounds, AEBSF, alpha-1-antitrypsin, Aprotinin, DFP, PMSF, TPCK and a-2-Macroglobulin
Form Crystallized, Lyophilized
Product Type alpha-Chymotrypsin
Molecular Weight (g/mol) 25
Color White
Concentration 1X
For Use With (Application) Catalyzes the hydrolysis of Peptide bonds involving L-isomers of Tyrosine, Phenylalanine, Tryptophan
Source Bovine pancreas
95

Thermo Scientific Chemicals Peroxidase, horseradish

Used in the oxidation of luminol to 3-aminophthalate. It is often used in conjugates to determine the presence of a molecular target, especially in the detection of specific protein in a western blot after conjugation with antibody.

Form Lyophilized
96

Thermo Scientific Chemicals Collagenase, Type I, Clostridium histolyticum

Type I Collagenase (CAS # 9001-12-1) is one of seven collagenases, enzymes responsible for collagen degradation.

Form Powder
97

MP Biomedicals™ Catalase

Fungal suspension from Aspergillus niger

Content And Storage 4°C
Form Liquid
Product Type Catalase
Molecular Weight (g/mol) ≈250
Concentration ≥1000 U/mL
For Use With (Application) Removal of Peroxides, the generation of Oxygen and in coupled systems, for the determination of metabolites
Source Aspergillus niger
98

MP Biomedicals™ Pancreatin 8X, USP Grade

From porcine pancreas

Content And Storage 0°C
Form Powder
Product Type Pancreatin
Color Cream
Concentration 8X
For Use With (Application) Promote esophageal tissue loosening in patients with esophageal tumors
Source Porcine pancreas
99

MP Biomedicals™ Cellulase from Aspergillus niger

Cellulase from Aspergillus niger catalyzes hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan and cellooligosaccharides cellotriose to cellohexaose

Content And Storage 4°C
Form Powder
Product Type Cellulase
Color Tan
pH 4.2 to 5.2
Concentration >60,000 U/g
For Use With (Application) Cellulase is used for commercial food processing in coffee, textile industry and in laundry detergents, pharmaceutical applications, fermentation of biomass into biofuels
Source Aspergillis niger
100

MP Biomedicals™ Proteinase K from Tritirachium album

Highly active stable endopeptidase with a broad spectrum of action

Content And Storage 4°C
Inhibitors Diisopropylfluorophosphate or Phenylmethane Sulfonyl Fluoride, AEBSF and Trypsin
Form Lyophilized Powder
Product Type Proteinase K
Molecular Weight (g/mol) 30000
Color White
pH 7.5 to 12
Grade Molecular Biology
For Use With (Application) Useful for the proteolytic inactivation of Nucleases during the isolation of DNA and RNA
Source Tritirachium album
101

Thermo Scientific Chemicals Pectinase, Aspergillus niger

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.

Form Powder
102

Lipase, MP Biomedicals™

Catalyze the hydrolysis and synthesis of the emulsified esters of glycerol and long chain fatty acids. Lipase, MP Biomedicals is a subclass of the esterases. It is used industrially for the resolution of chiral compounds and the trans-esterification produ

103

MP Biomedicals™ Collagenase, Type 4

From Clostridium histolyticum

Content And Storage 4°C
Inhibitors Cysteine, EDTA or o-phenanthroline, alpha2-macroglobulin - a large plasma glycoprotein, alpha2-macroglobulin, alpha1-antitrypsin
Form Lyophilized Powder
Product Type Collagenase Type 4
Color Brown
Concentration >160 U/mg
For Use With (Application) For tissue dissociation combined with other Enzymes such as Elastase, Trypsin/Papain
Source Clostridium histolyticum
104

MP Biomedicals™ Ficin

From fig tree

Content And Storage 4°C
Inhibitors Iodoacetamide, Iodoacetic Acid, N-ethylmaleimide, Mercuric Chloride, DFP (Diisopropyl Fluorophosphate), TLCK (Na-p-Tosyl-lysine Chloromethyl Ketone), TPCK (N-Tosyl-L-phenylalanine Chloromethyl Ketone)
Form Powder
Product Type Ficin
Molecular Weight (g/mol) ≈25
Color White
pH 5 to 7.5
For Use With (Application) Cleavage of Mouse IgG
Source Fig tree Ficus glabrata
105

MP Biomedicals™ Hyaluronidase, From Streptomyces hyalurolyticus

Enzyme activity is determined by a change in optical density in a hyaluronic acid solution.

Inhibitors Mn2+, Hg2+, K+ Ferricyanide
Form Lyophilized Powder
Product Type Hyaluronidase
Color White
pH 6
Concentration ≈2000 U/mg
Source Streptomyces hyalurolyticus